BioVIS Lab. in BioVIS Technology Center of NASA Ames Research Center  is dedicated to developing and applying advanced visualization, computation, and simulation technologies to support NASA Space Life Sciences research and the objectives of the Fundamental Biology Program. On-going virtual environment technology development at CBI is directed toward development of a virtual simulator (Virtual GloveboX or VGX) to provide an advanced "fine-motor coordination" training and simulation system for astronauts to perform precise biological experiments in a glovebox aboard the International Space Station. Effectiveness of the VGX as a training tool depends both on precision of the sensed motion and ease of use. The present design of the VGX utilizes research technologies that currently incorporate electro-mechanical sensing gloves and force feedback (or haptic) devices, which has inherit limitations in terms of maintaining the naturalness of user interaction with the simulator. To enhance the utility and effectiveness of the VGX, we will develop and demonstrate versatile, non-contact, computer vision-based interaction techniques for real-time tracking and registration of the human arm, hand, and fingers in space. To improve the effectiveness of the interaction of the astronauts with the VGX, we will develop and demonstrate algorithms to visually estimate head and eye (gaze) direction in space. Our rationale is that gaze information has the potential to indicate intention and subsequent movement. Moreover, multi-scale resolution algorithms can be applied to detail the desired visual targets, reducing the resolution of peripheral visual images, and thereby increasing speed for real-time interactive procedures and mimicking human visual perception.