\documentclass[12pt]{seminar}
\usepackage[dvips]{pstcol} % To use the standard `color' package with Seminar
\usepackage{multicol}
\usepackage{amsbsy}
\usepackage{amssymb}
\usepackage{amsmath}
\usepackage{amsfonts}
\usepackage{semcolor}
\usepackage{semhelv}
\usepackage{fancybox}
\usepackage{graphicx}
\usepackage{natbib}
\usepackage{semlayer}
\input{seminar.bug}
\input{seminar.bg2} % See the Seminar bugs list
\usepackage{pst-grad}
% Definition of new colors
\definecolor{Beige}   {rgb}{0.96,0.96,0.86}
%\definecolor{DarkerBeige}   {rgb}{0.98,0.98,0.75}
\definecolor{Gold}    {rgb}{1.,0.84,0.}
\definecolor{MyYellow}{rgb}{1.,0.84,0.8}
\definecolor{myblue}{rgb}{0.074509804,0.470588235,1.0}
%\definecolor{verylightgray}{rgb}{0.9,0.9,0.9}
\definecolor{lightgray}{rgb}{0.7,0.7,0.7}
%\slideframe[\psset{fillstyle=gradient,gradbegin=myblue,gradend=white,gradmidpoint=1}]{scplain}
\slideframe[\psset{fillstyle=gradient,gradbegin=myblue,gradend=white,gradmidpoint=1,linearc=.01mm,linewidth=0.1mm}]{scplain}
\renewcommand{\printlandscape}{\special{papersize=297mm,223mm}}
%\slidewidth 276mm
\slidewidth 281mm
%\slideheight 203mm
\slideheight 207.1mm
\renewcommand{\slideleftmargin}{3.25cm}
%\renewcommand{\slidetopmargin}{0.025cm}
\renewcommand{\slidetopmargin}{-0.77mm}
\bibliographystyle{apalike}

% ============================================================================

\begin{document}
%INSIGHT SLIDE
\begin{slide}
  \centerline{\psframebox[fillstyle=solid,fillcolor=blue,framearc=.1,framesep=2mm]{\large\bfseries\color{yellow}Title}}
\begin{multicols}{2}
\textbf{subject}
  \begin{itemize}
     \item one
     \item two 
     \item three 
     \item four 
  \end{itemize}
\textbf{subject}
  \begin{itemize}
     \item one
     \item two
     \item three
     \item four
  \end{itemize}
\end{multicols}
\end{slide}
\end{document}